Reduced expression of CAMTA1 by exercise mediate lipid oxidation

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doi:2014.22.1.95

Exerc Sci > Volume 22(1); 2014 > Article

Exercise Science 2014;22(1): 95-102. doi: https://doi.org/10.15857/ksep.2014.22.1.95

운동에 의한 CAMTA1의 발현 감소가 간세포의 지방산화에 미치는 영향

문효열¹, 박성태², 김기정³

¹울산과학기술대
²경주대학교
³서울대학교

Reduced expression of CAMTA1 by exercise mediate lipid oxidation

Published online: December 14, 2015.

ABSTRACT

Exercise is known to have an ameliorating effect on chronic metabolic diseases such as obesity, diabetes and nonalcoholic fatty liver disease(NAFLD), yet the underlying mechanism is not fully understood. To identify the reduced expression of genes by exercise and to characterize the role of this gene in lipid metabolism, we first identified the one of candidate gene,Calmodulin-binding transcription activator 1 (CAMTA 1) which showed reduced- mRNA expression after exercise from GEO datasets. Then we confirmed the expression of this candidate gene, CAMTA1, was decreased by 4 week treadmill experiment. Transfection of CAMTA1siRNA increased phosphorylation of AMPK and ACC in the HepG2, human hepatoblastoma cell line. This was accompanied by increases of palmitate oxidation in CAMTA1 siRNA transfected cell lines compared to their control cells. Furthermore, AMPK and CaMKK pathway mediate the knockdown of CAMTA1-induced palmitate oxidation. Taken together, these results suggest a novel role of CAMTA1 in mediating the lipid oxidation of exercise, probably via AMPK pathway.

Key words: Treadmill exercise, Camta-1, Fatty liver