| 운동에 의한 CAMTA1의 발현 감소가 간세포의 지방산화에 미치는 영향 |
| 문효열1, 박성태2, 김기정3 |
1울산과학기술대
2경주대학교
3서울대학교 |
| Reduced expression of CAMTA1 by exercise mediate lipid oxidation |
|
|
|
Published online: December 14, 2015. |
|
|
|
| ABSTRACT |
|
Exercise is known to have an ameliorating effect on chronic metabolic diseases such as obesity, diabetes and nonalcoholic fatty liver disease(NAFLD), yet the underlying mechanism is not fully understood. To identify the reduced expression of genes by exercise and to characterize the role of this gene in lipid metabolism, we first identified the one of candidate gene,Calmodulin-binding transcription activator 1 (CAMTA 1) which showed reduced- mRNA expression after exercise from GEO datasets. Then we confirmed the expression of this candidate gene, CAMTA1, was decreased by 4 week treadmill experiment. Transfection of CAMTA1siRNA increased phosphorylation of AMPK and ACC in the HepG2, human hepatoblastoma cell line. This was accompanied by increases of palmitate oxidation in CAMTA1 siRNA transfected cell lines compared to their control cells. Furthermore, AMPK and CaMKK pathway mediate the knockdown of CAMTA1-induced palmitate oxidation. Taken together, these results suggest a novel role of CAMTA1 in mediating the lipid oxidation of exercise, probably via AMPK pathway. |
| Key words:
Treadmill exercise, Camta-1, Fatty liver |
|
|
|
PDF Links
Full text via DOI
Download Citation 
