Mitochondrial Biosynthesis Factors of Offspring before and during Maternal Training Exercise in Rat Skeletal Muscle

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doi:2014.23.2.69

Exerc Sci > Volume 23(2); 2014 > Article

Exercise Science 2014;23(2): 69-77. doi: https://doi.org/10.15857/ksep.2014.23.2.69

수태 전과 수태 중 모체운동 훈련이 어미 쥐와 새끼 쥐의 골격근 세포 내 미토콘드리아 생합성 요소에 미치는 영향

전유나, 권형태, 김형준, 여효성, 김효정, 김창근

한국체육대학교

Mitochondrial Biosynthesis Factors of Offspring before and during Maternal Training Exercise in Rat Skeletal Muscle

ABSTRACT

The present study was to investigate whether mitochondrial biosynthetic markers of offspring is affected by maternal exercise training during pregnancy. Twenty-one SD rats (10 wks) were randomly assigned into CON (control, n=7), EBP (exercise before pregnancy, n=7) and EDP (exercise during pregnancy, n=7) group. Exercise groups ran on the rodent treadmill at 60-75 %VO2max for 10 weeks (1 h/d, 5 d/wk). Muscle samples were obtained from offsprings at immediately after and 8 weeks after birth, and mother rats were also sacrificed 4 weeks after delivering the offspring. Mitochondrial biosynthetic markers, COX-Ⅳ, mtTFA, PGC-1α proteins and angiogenesis factors, VEGF165, VEGFtotal, HIF-1α proteins were measured by western blotting. The expression of maternal PGC-1α, mtTFA, and COX-Ⅳ proteins in both EBP and EDP groups were significantly upregulated than CON group after aerobic exercise training. The expression of maternal VEGF165, VEGFtotal were significantly upregulated than CON group after aerobic exercise training, whereas no difference in HIF-1α protein level were found among the groups. The expression of COX-Ⅳ, mtTFA, PGC-1α protein levels of offsprings in EDP group were similar to maternal values and significantly higher than EBP and CON groups immediately after birth and maintained up to 8 weeks after birth. The trend of angiogenesis factors, VEGF165, VEGFtotal, HIF-1α proteins of offsprings also similar to mitochondrial biosynthetic markers immediately after and 8 weeks after birth as well. In summary, prolonged and regular maternal aerobic exercise during pregnancy increases mitochondrial biosynthetic and angiogenetic factors in skeletal muscles in both maternal and offsprings. Furthermore, prolonged and regular maternal aerobic exercise during pregnancy maintains upregulated levels of mitochondrial biosynthetic and angiogenetic factors during growth and development of offsprings.

Key words: maternal exercise, mitochondrial biogenesis, angiogenesis