| 12주간의 고지방 식이가 지방내 혈관 함량에 미치는 영향 |
| 이혁종 |
| Cornell University |
| The effect of 12 weeks high fat diet treatment on adipose tissue blood vessel content |
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| ABSTRACT |
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Adipose tissue specific ablation of VEGF induces less vasculature in mouse epididymal fat(Epi. fat) and deteriorates inflammation and insulin resistance under high fat diet condition. But it has not been evaluated whether obesity itself directly regulates blood vessel content in adipose tissue yet. The purposes of this study are to identify blood vessel content between lean and obese Epi. fat and to provide a justification for the study about exercise training effect on fat vasculature in the future. For the analysis of vascular pattern in adipose tissue, Epi. fats of 1 day, 2 weeks, 3 weeks and 4 weeks of age were observed under confocal microscope after staining with PECAM-1. With near infrared fluorescence conjugated lectin and VE-cadherin antibody, blood vessel content was evaluated in Epi fat from the mice fed low (control) or high fat (obesity) diet for 12 weeks. Also mRNA expression of VEGF and angiopoietin were analyzed. At the postnatal one day, the filopodia, which indicated angiogenic process, was found in Epi. fat and strong angiogenic process was going on up to 3 weeks of age. Then the vessels had uniformed size and structure at 4 weeks of age. The lectin fluorescence concentration was significantly decreased in obese Epi. fat (p<.05). Also VE-cadherin protein expression was significantly less in obese Epi. fat (p<.01). In parallel with less blood vessel content, VEGF mRNA expression in obese Epi. fat was significantly down-regulated (p<.01), but angiopoietin was up-regulated (p<.001). Correctively, these findings suggest that the obese Epi. fat has less blood vessel content by down regulation of VEGF mRNA. These results will be useful information for interrogating the effect of exercise on obese fat blood vessel content in the future. |
| Key words:
Obesity, High fat diet, Vasculature content, Epididymal fat, VE-cadherin |
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