| 지방내 crown-like-structure 대식세포의 새로운 표식인자로써 LYVE-1의 역할 |
| 이혁종 |
| Cornell University |
| LYVE-1 as a novel selection marker for crown like structure macrophage in adipose tissue. |
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| ABSTRACT |
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Although exercise training improves adipose tissue associated inflammation and systemic insulin resistance in obese patients, the underlying mechanisms how exercise effects on macrophage functions in obese adipose tissue have not been evaluated yet. The main reason for this is the absence of specific marker for isolating two different macrophages, resident macrophage(RM) and crown-like-structure macrophage(CLSM), from obese adipose tissue. Therefore, the purpose of this study is to identify a new phenotypic marker for the selection of RM and CLSM and to provide useful method for studying the effects of exercise training on adiopose tissue macrophage. Pro-inflammatory cytokine expression, blood vessel activation, and ATM recruitment were analyzed in the adipose tissues from the mice fed low(control) or high fat diet(obesity) for 12 weeks. Also novel marker for detection of CLSM was evaluated with LYVE-1 staining in this study. TNF-α(p<.001), IL-1b(p<.01) and Cox-2(p<.05) were upregulated in obese adipose tissue. ICAM-1(p<.01) and VCAM-1(p<.001), which were known to play a role in recruiting leukocyte into adipose tissue, were highly expressed on adipose tissue blood vessel in obese group and more RM and CLSM were accumulated in obese adipose tissue. To identify a new marker for the detection of CLSM, MGL1 and LYVE-1, which were M2 markers, were evaluated. The expression of MGL1, which has been known not to be expressed on CLSM, was found on CLSM. It indicates that MGL1 is not an ideal marker for selection of CLSM. Instead, LYVE-1 was expressed on RM in lean and obese adipose tissue RM, but not in CLSM. Therefore, CLSM is redefined as F4/80+CD11c+LYVE-1-. This new novel marker would be useful for isolating and characterizing CLSM as well as for interrogating the effects of endurance exercise on adipose tissue macrophage in obese patients. |
| Key words:
Obesity, Epididymal fat, Crown like structure, Macrophage, LYVE-1 |
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