Effect of exercise training on protein expression of PPARδ and PGC-1α in obese Zucker rat skeletal muscles.

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Exerc Sci > Volume 14(2); 2005 > Article

Exercise Science 2005;14(2): 109-120.

지구성 운동이 비만 쥐 골격근의 PPARδ와 PGC-1α의 발현 및 효소 활성도에 미치는 영향

신영오¹, 강호율², 오재근¹, 김영주¹, 박수연¹, 김호성¹, 권기욱¹, 이종삼³

¹한국체육대학교
²경북대학교
³을지의과대학교

Effect of exercise training on protein expression of PPARδ and PGC-1α in obese Zucker rat skeletal muscles.

ABSTRACT

Shin, Y.O., Lee, J.S., Park, S.Y., Kang, H.Y., Kim, Y.J., Kim, H.S., Kwon, K.W., Oh, J.K. Effect of exercise training on protein expression of PPARδ and PGC-1α in obese Zucker rat skeletal muscles. Exercise Science, 14(2): 109-120, 2005. Recent emerging evidences suggested that peroxisome proliferator-activated receptor δ (PPARδ), and peroxisome proliferator-activated receptorγ coactivator 1α (PGC-1α) play a pivotal role in fatty acid oxidation. Present study investigated regulatory effects of exercise training on PPARδ and PGC-1α in insulin resistance obese Zucker rats. Investigation was further extended in two different (soleus, SOL(type I) vs. extensor digitorum logus, EDL(type II)) muscle fiber types, mitochondrial oxidative enzyme activity were analysed. After 8 weeks of treadmill running procedure, oral glucose tolerance test (OGTT; 2gkg-1 body mass; BM) were underwent in all groups(lean Zucker control rats; LN, obese Zucker control rats; OB, exercise-trained obese Zucker rats; EXE) and blood glucose, insulin, and free fatty acids levels were measured. Exercise training significantly improved insulin sensitivity and blood lipid profiles. Activity of citrate synthase(CS) and β-Hydroxyacyl-CoA Dehydrogenase (β-HAD) in SOL was increased significantly, but not in EDL. PGC-1α protein contents was not different between OB and LN in both of muscle fiber types. However, PPARδ protein contents was significantly deceased in SOL of OB than LN. Exercise training significantly elevated protein expression of PGC-1α and PPARδ from OB in both muscle fiber types. These findings indicate that adaptation of skeletal muscles to exercise training increased in protein expression of PPARδ and PGC-1α associated with fatty acid oxidation.

Key words: obesity, exercise, insulin resistance, oral glucose tolerance test, muscle fiber types, PPARδ, PGC-1α.