| 운동강도의 차이가 대장에서의 Heme oxygenase-1 발현에 미치는 영향 : Oligonucleotide chip microarray analysis |
| 최은주, 류호영, 차광석 |
| 건국대학교 |
| Effect of exercise intensity patterns training on heme oxyganase-1 expression in large intestine. |
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| ABSTRACT |
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The purpose of this study is to identify how large intestine tissue effects to mRNA expression in Heme oxygenase-1 (HO-1) due to differences in exercise intensity. To perform this experiment, we divided Sprage-Dawley rats into 3 groups (control group (CON), low-intensity exercise group (LIE) and high-intensity exercise group (HIE)), and in 48 hours after 4 weeks treadmill exercise, the following experiment was performed with “High through-put microarray analysis methods”. 12,079 genes were chosen by filtering of the Rat ABI oligo chip 26,857 genes in this experiment. Among 12,079 genes, we selected significant genes (p<.05) that were also expressed in the ways of clustering analysis and immunohistochemistry. With results of RT-PCR confirming and microarray analyzing, we derived that gene expression profiles of HO-1 had been consistent. As a result of this experiment, we certainly identified that exercise effects to HO-1 expression in large intestine. mRNA expression was more expressed in high-intensity exercise group (HIE) than low-intensity exercise group (LIE), it seems that homeostasis maintains itself by properly removing oxygen radical, caused by high intensity exercise, through reduction of HO-1. |
| Key words:
Microarray, Large intestine, Heme oxygenase-1 |
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